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multimacs cell24 separator plus  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec multimacs cell24 separator plus
    Multimacs Cell24 Separator Plus, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multimacs+cell24+separator+plus/MultiMACS+Cell24+Separator+Plus/bio_rxiv__64898__2026__01__07__697678-120-27-31
    Average 95 stars, based on 35 article reviews
    multimacs cell24 separator plus - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
    Article Snippet: Pups (P4-P7) were euthanized by decapitation, and brains, cortices, and corpora callosa were dissected and dissociated using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) with the gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). .. OPCs were isolated via magnetic cell sorting (MACS) using anti-PDGFRα-coupled-beads (CD140a-PDGFRα MicroBead Kit, Miltenyi Biotec, 130-101-502) and the MultiMACS Cell24 Separator Plus (Miltenyi Biotec, 130-095-691). .. 40,000 cells were plated on coverslips pre-coated with poly-L-ornithine (sigma, P4957) and amplified for two/three days in a proliferating medium containing DMEM High Glucose (Dutscher, L0103-500), Ham′s Nutrient Mixture F12 (Merck, 51651 C), L-Glutamine (Thermo, 200 mM), Hormone mix (Gritti, A., et al., 2001), Penicillin/Streptomycin (life technology, 15140122, 1X), 20 ng/ml EGF (Peprotech, AF-100-15), 10 ng/ml FGF-basic (Peprotech, 100-18B), and 10 ng/ml PDGF-AA (PeproTech, 100-13 A).

    Article Title: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
    Article Snippet: brains from P4 mice, cortices, and corpora callosa were dissected and dissociated using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) with the gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). .. OPCs were isolated via magnetic cell sorting (MACS) using anti-PDGFRα-coupled-beads (CD140a-PDGFRα MicroBead Kit, Miltenyi biotec, 130-101-502) and the MultiMACS Cell24 Separator Plus (Miltenyi biotec, 130-095-691). .. 40,000 cells were plated on coverslips pre-coated with poly-L-ornithine (sigma, P4957) and amplified for two/three days in a proliferating medium containing DMEM High Glucose (Dutscher, L0103-500), Ham′s Nutrient Mixture F12 (Merck, 51651C), L-Glutamine (Thermo, 200 mM), Hormone mix (Gritti, A., et al., 2001), Penicillin/Streptomycin (life technology, 15140122, 1X), 20ng/ml EGF (Peprotech, AF-100-15), 10ng/ml FGF-basic (Peprotech, 100-18B), and 10ng/ml PDGF-AA (PeproTech, 100-13A).

    Article Title: Immunogenicity of Non-Mutated Ovarian Cancer-Specific Antigens
    Article Snippet: .. Peripheral Blood Mononuclear Cells (PBMCs) were then isolated using Ficoll Gradient (Cytiva, Vancouver, BC, Canada, #cat.17144003) or the MultiMACS Cell24 Separator Plus (Miltenyi Biotec, #cat. 130-098-637) in conjugation with the Straight From Leukopak PBMC Isolation Kit (Miltenyi Biotec, #cat. 130-123-456) according to the manufacturer’s protocol. ..

    Article Title: Increased functional potency of multi-edited CAR-T cells manufactured by a non-viral transfection system
    Article Snippet: Leukopaks (BioIVT, HUMANLMX100-0001129) were obtained from de-identified and healthy human donors. .. T cells were isolated from the fresh Leukopak, within 24 h of collection, via positive selection using the Straight from Leukopak CD4 (Miltenyi Biotec, 130117022) and CD8 (Miltenyi Biotec, 130117019) Microbead kits and MultiMACS Cell24 Separator Plus (Miltenyi Biotec 130-098-637), according to the manufacturer’s protocol. .. Briefly, CD4/CD8-labeled Leukopak samples were loaded onto the Multi-24 Column Block (Miltenyi Biotec, 130-095-692), where, after removing the column block from the magnetic field, the magnetically retained CD4 + /CD8 + T cells were eluted as a positively selected cell fraction.

    FACS:

    Article Title: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
    Article Snippet: Pups (P4-P7) were euthanized by decapitation, and brains, cortices, and corpora callosa were dissected and dissociated using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) with the gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). .. OPCs were isolated via magnetic cell sorting (MACS) using anti-PDGFRα-coupled-beads (CD140a-PDGFRα MicroBead Kit, Miltenyi Biotec, 130-101-502) and the MultiMACS Cell24 Separator Plus (Miltenyi Biotec, 130-095-691). .. 40,000 cells were plated on coverslips pre-coated with poly-L-ornithine (sigma, P4957) and amplified for two/three days in a proliferating medium containing DMEM High Glucose (Dutscher, L0103-500), Ham′s Nutrient Mixture F12 (Merck, 51651 C), L-Glutamine (Thermo, 200 mM), Hormone mix (Gritti, A., et al., 2001), Penicillin/Streptomycin (life technology, 15140122, 1X), 20 ng/ml EGF (Peprotech, AF-100-15), 10 ng/ml FGF-basic (Peprotech, 100-18B), and 10 ng/ml PDGF-AA (PeproTech, 100-13 A).

    Article Title: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
    Article Snippet: brains from P4 mice, cortices, and corpora callosa were dissected and dissociated using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) with the gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). .. OPCs were isolated via magnetic cell sorting (MACS) using anti-PDGFRα-coupled-beads (CD140a-PDGFRα MicroBead Kit, Miltenyi biotec, 130-101-502) and the MultiMACS Cell24 Separator Plus (Miltenyi biotec, 130-095-691). .. 40,000 cells were plated on coverslips pre-coated with poly-L-ornithine (sigma, P4957) and amplified for two/three days in a proliferating medium containing DMEM High Glucose (Dutscher, L0103-500), Ham′s Nutrient Mixture F12 (Merck, 51651C), L-Glutamine (Thermo, 200 mM), Hormone mix (Gritti, A., et al., 2001), Penicillin/Streptomycin (life technology, 15140122, 1X), 20ng/ml EGF (Peprotech, AF-100-15), 10ng/ml FGF-basic (Peprotech, 100-18B), and 10ng/ml PDGF-AA (PeproTech, 100-13A).

    Magnetic Cell Separation:

    Article Title: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
    Article Snippet: Pups (P4-P7) were euthanized by decapitation, and brains, cortices, and corpora callosa were dissected and dissociated using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) with the gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). .. OPCs were isolated via magnetic cell sorting (MACS) using anti-PDGFRα-coupled-beads (CD140a-PDGFRα MicroBead Kit, Miltenyi Biotec, 130-101-502) and the MultiMACS Cell24 Separator Plus (Miltenyi Biotec, 130-095-691). .. 40,000 cells were plated on coverslips pre-coated with poly-L-ornithine (sigma, P4957) and amplified for two/three days in a proliferating medium containing DMEM High Glucose (Dutscher, L0103-500), Ham′s Nutrient Mixture F12 (Merck, 51651 C), L-Glutamine (Thermo, 200 mM), Hormone mix (Gritti, A., et al., 2001), Penicillin/Streptomycin (life technology, 15140122, 1X), 20 ng/ml EGF (Peprotech, AF-100-15), 10 ng/ml FGF-basic (Peprotech, 100-18B), and 10 ng/ml PDGF-AA (PeproTech, 100-13 A).

    Article Title: Antagonistic actions of PAK1 and NF2/Merlin drive myelin membrane expansion in oligodendrocytes.
    Article Snippet: Sorbonne Université, Institut du Cerveau, Paris Brain Institute ICM, Inserm, CNRS, APHP, Hôpital de la Pitié-Salpêtrière, Paris, France Al-Quds University, Faculty of Medicine, Jerusalem, Palestine Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, Université de Paris, Paris, France Sorbonne Université, Inserm, UMS Production et Analyse des Données en Sciences de la vie et en Santé, PASS, Plateforme Post-génomique de la PitiéSalpêtrière, Paris, France Institut des Neurosciences Paris-Saclay, UMR 9197, CNRS, Université Paris-Saclay, Saclay, France

    Article Title: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
    Article Snippet: brains from P4 mice, cortices, and corpora callosa were dissected and dissociated using a neural tissue dissociation kit (P) (Miltenyi Biotec, 130-092-628) with the gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). .. OPCs were isolated via magnetic cell sorting (MACS) using anti-PDGFRα-coupled-beads (CD140a-PDGFRα MicroBead Kit, Miltenyi biotec, 130-101-502) and the MultiMACS Cell24 Separator Plus (Miltenyi biotec, 130-095-691). .. 40,000 cells were plated on coverslips pre-coated with poly-L-ornithine (sigma, P4957) and amplified for two/three days in a proliferating medium containing DMEM High Glucose (Dutscher, L0103-500), Ham′s Nutrient Mixture F12 (Merck, 51651C), L-Glutamine (Thermo, 200 mM), Hormone mix (Gritti, A., et al., 2001), Penicillin/Streptomycin (life technology, 15140122, 1X), 20ng/ml EGF (Peprotech, AF-100-15), 10ng/ml FGF-basic (Peprotech, 100-18B), and 10ng/ml PDGF-AA (PeproTech, 100-13A).

    Single Cell:

    Article Title: Single-antibody mutational scanning reveals poly-specificity and immune constraints in vaccination responses
    Article Snippet: .. The single-cell suspensions were centrifuged at 4°C, 400 g for 5 min, and washed in buffer twice before using the murine Pan B cell isolation kit and MultiMACS Cell24 Separator Plus (Miltenyi Biotec) according to the manufacturer’s instructions. ..

    Cell Isolation:

    Article Title: Single-antibody mutational scanning reveals poly-specificity and immune constraints in vaccination responses
    Article Snippet: .. The single-cell suspensions were centrifuged at 4°C, 400 g for 5 min, and washed in buffer twice before using the murine Pan B cell isolation kit and MultiMACS Cell24 Separator Plus (Miltenyi Biotec) according to the manufacturer’s instructions. ..

    Conjugation Assay:

    Article Title: Immunogenicity of Non-Mutated Ovarian Cancer-Specific Antigens
    Article Snippet: .. Peripheral Blood Mononuclear Cells (PBMCs) were then isolated using Ficoll Gradient (Cytiva, Vancouver, BC, Canada, #cat.17144003) or the MultiMACS Cell24 Separator Plus (Miltenyi Biotec, #cat. 130-098-637) in conjugation with the Straight From Leukopak PBMC Isolation Kit (Miltenyi Biotec, #cat. 130-123-456) according to the manufacturer’s protocol. ..

    Selection:

    Article Title: Increased functional potency of multi-edited CAR-T cells manufactured by a non-viral transfection system
    Article Snippet: Leukopaks (BioIVT, HUMANLMX100-0001129) were obtained from de-identified and healthy human donors. .. T cells were isolated from the fresh Leukopak, within 24 h of collection, via positive selection using the Straight from Leukopak CD4 (Miltenyi Biotec, 130117022) and CD8 (Miltenyi Biotec, 130117019) Microbead kits and MultiMACS Cell24 Separator Plus (Miltenyi Biotec 130-098-637), according to the manufacturer’s protocol. .. Briefly, CD4/CD8-labeled Leukopak samples were loaded onto the Multi-24 Column Block (Miltenyi Biotec, 130-095-692), where, after removing the column block from the magnetic field, the magnetically retained CD4 + /CD8 + T cells were eluted as a positively selected cell fraction.



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